Nicola Crosetto

About me
Publications
Public outreach and news

About me

I graduated in Medicine at the University of Pavia, Italy in 2003 and
specialized in Medical Oncology at the University of Turin, Italy in 2007.
From 2008 until 2010, I worked in Ivan Dikic’s lab at the Goethe University
in Frankfurt, Germany and in 2011 I obtained a PhD in Bioengineering and
Bioinformatics at the University of Pavia, Italy. From 2011 until 2014 I was
a postdoc in Alexander van Oudenaarden’s lab at MIT, where I worked on
developing novel single-molecule microscopy methods. Since 2015, I have been
leading my own research group at ̽��ѡ, with a major focus on
developing microscopy- and sequencing-based methods to investigate genome
fragility and genome organization in healthy and pathological cell states.
Since 2022 I also lead the Genomic Technologies Development Unit in the
Genomics Research Centre at Human Technopole in Milan, Italy.
Over the years, I have developed numerous molecular methods, including the
first method for genome-wide mapping of DNA double-strand breaks (BLESS, /Nat
Meth/ 2013) and its downstream improvements (BLISS, /Nat Commun/ 2017
sBLISS, /Nat Prot/ 2020), as well as a variety of sequencing- (CUTseq, /Nat
Commun/ 2019
GPSeq, /Nat Biotechnol/ 2020
COVseq, /Nat Commun/ 20221) and
microscopy-based methods (HD-FISH, /Nat Meth/ 2013
FuseFISH, /Cell Rep/
2014
RollFISH, /Comm Bio/ 2018
iFISH, /Nat Commun/ 2019
FRET-FISH, /Nat
Commun/ 2022) for studying various aspects of genome organization and
fragility.

Research

Our main research interest is in understanding how the nucleus of eukaryotic
cells is spatially organized, and how the three-dimensional (3D) architecture
of the genome influences gene expression regulation and genome integrity.
We are particularly interested in understanding the role of 3D genome
architecture and genome fragility in the context of cancer, aiming at
developing improved diagnostic and patient stratification approaches.
Towards these goals, we combine high-end microscopy methods, next-generation
sequencing techniques, and advanced computational tools, to assess
chromosomal contacts, chromatin modifications, DNA breaks, chromosomal
rearrangements and gene expression levels in cultured cells and
patient-derived tissue samples.

Articles

Article: NATURE COMMUNICATIONS. 2024;15(1):3475

Zhang N; Harbers L; Simonetti M; Diekmann C; Verron Q; Berrino E; Bellomo SE; Longo GMC; Ratz M; Schultz N; Tarish F; Su P; Han B; Wang W; Onorato S; Grassini D; Ballarino R; Giordano S; Yang Q; Sapino A; Frisen J; Alkass K; Druid H; Roukos V; Helleday T; Marchio C; Bienko M; Crosetto N
Article: NATURE COMMUNICATIONS. 2024;15(1):1768

Chen JP; Diekmann C; Wu H; Chen C; Della Chiara G; Berrino E; Georgiadis KL; Bouwman BAM; Virdi M; Harbers L; Bellomo SE; Marchio C; Bienko M; Crosetto N
Article: SCIENCE ADVANCES. 2023;9(45):eadi2095

Chatzinikolaou G; Stratigi K; Siametis A; Goulielmaki E; Akalestou-Clocher A; Tsamardinos I; Topalis P; Austin C; Bouwman BAM; Crosetto N; Altmueller J; Garinis GA
Article: BIOLOGY OPEN. 2023;12(10):bio060113

Mastropasqua F; Oksanen M; Soldini C; Alatar S; Arora A; Ballarino R; Molinari M; Agostini F; Poulet A; Watts M; Rabkina I; Becker M; Li D; Anderlid B-M; Isaksson J; Remnelius KL; Moslem M; Jacob Y; Falk A; Crosetto N; Bienko M; Santini E; Borgkvist A; Bo''lte S; Tammimies K
Article: CURRENT OPINION IN GENETICS AND DEVELOPMENT. 2023;78:102020

Bouwman BAM; Crosetto N; Bienko M
Article: JTO CLINICAL AND RESEARCH REPORTS. 2022;3(12):100435

Nicos M; Harbers L; Patrucco E; Kramer-Drauberg M; Zhang X; Voena C; Kowalczyk A; Bozyk A; Peksa R; Jarosz B; Szumilo J; Simonetti M; Zuk M; Wasag B; Reszka K; Duchnowska R; Milanowski J; Chiarle R; Bienko M; Krawczyk P; Jassem J; Ambrogio C; Crosetto N
Article: NATURE COMMUNICATIONS. 2022;13(1):6680

Mota A; Berezicki S; Wernersson E; Harbers L; Li-Wang X; Gradin K; Peuckert C; Crosetto N; Bienko M
Article: BMC CANCER. 2022;22(1):1001

Raczkowski L; Pasnik I; Kukielka M; Nicos M; Budzinska MA; Kucharczyk T; Szumilo J; Krawczyk P; Crosetto N; Szczurek E
Article: GENOME MEDICINE. 2022;14(1):98

Berrino E; Annaratone L; Bellomo SE; Ferrero G; Gagliardi A; Bragoni A; Grassini D; Guarrera S; Parlato C; Casorzo L; Panero M; Sarotto I; Giordano S; Cereda M; Montemurro F; Ponzone R; Crosetto N; Naccarati A; Sapino A; Marchio C
Article: SCIENTIFIC DATA. 2022;9(1):400

Ballarino R; Bouwman BAM; Agostini F; Harbers L; Diekmann C; Wernersson E; Bienko M; Crosetto N
Article: SCIENTIFIC DATA. 2022;9(1):47

Mota A; Schweitzer M; Wernersson E; Crosetto N; Bienko M
Article: CURRENT OPINION IN CELL BIOLOGY. 2022;74:7-12

Bouwman BAM; Crosetto N; Bienko M
Article: MOLECULAR CELL. 2021;81(24):5007-5024.e9

Wiegard A; Kuzin V; Cameron DP; Grosser J; Ceribelli M; Mehmood R; Ballarino R; Valant F; Grochowski R; Karabogdan I; Crosetto N; Lindqvist A; Bizard AH; Kouzine F; Natsume T; Baranello L
Article: MOLECULAR CELL. 2021;81(23):4907-4923.e8

Zampetidis CP; Galanos P; Angelopoulou A; Zhu Y; Polyzou A; Karamitros T; Kotsinas A; Lagopati N; Mourkioti I; Mirzazadeh R; Polyzos A; Garnerone S; Mizi A; Gusmao EG; Sofiadis K; Gal Z; Larsen DH; Pefani D-E; Demaria M; Tsirigos A; Crosetto N; Maya-Mendoza A; Papaspyropoulos A; Evangelou K; Bartek J; Papantonis A; Gorgoulis VG
Article: NPJ BREAST CANCER. 2021;7(1):144

Zerdes I; Simonetti M; Matikas A; Harbers L; Acs B; Boyaci C; Zhang N; Salgkamis D; Agartz S; Moreno-Ruiz P; Bai Y; Rimm DL; Hartman J; Mezheyeuski A; Bergh J; Crosetto N; Foukakis T
Article: NATURE COMMUNICATIONS. 2021;12(1):3903

Simonetti M; Zhang N; Harbers L; Milia MG; Brossa S; Nguyen TTH; Cerutti F; Berrino E; Sapino A; Bienko M; Sottile A; Ghisetti V; Crosetto N
Article: VACCINES. 2021;9(3):235

Merlano MC; Abbona A; Paccagnella M; Falletta A; Granetto C; Ricci V; Fea E; Denaro N; Ruatta F; Merlotti A; Bertetto O; Crosetto N; Galizia D; Basirico M; Gammaitoni L; Sangiolo D; Aglietta M; Garrone O
Article: FRONTIERS IN ONCOLOGY. 2021;11:700568

Harbers L; Agostini F; Nicos M; Poddighe D; Bienko M; Crosetto N
Article: METHODS IN MOLECULAR BIOLOGY. 2021;2162:261-281

Ballarino R; Bouwman BAM; Crosetto N
Article: NATURE PROTOCOLS. 2020;15(12):3894-3941

Bouwman BAM; Agostini F; Garnerone S; Petrosino G; Gothe HJ; Sayols S; Moor AE; Itzkovitz S; Bienko M; Roukos V; Crosetto N
Article: ESMO OPEN. 2020;5(5):e000876

Garrone O; Michelotti A; Paccagnella M; Montemurro F; Vandone AM; Abbona A; Geuna E; Vanella P; De Angelis C; Lo Nigro C; Falletta A; Crosetto N; Di Maio M; Merlano M
Article: NATURE BIOTECHNOLOGY. 2020;38(10):1184-1193

Girelli G; Custodio J; Kallas T; Agostini F; Wernersson E; Spanjaard B; Mota A; Kolbeinsdottir S; Gelali E; Crosetto N; Bienko M
Article: NATURE MEDICINE. 2020;26(7):1063-1069

Dziubanska-Kusibab PJ; Berger H; Battistini F; Bouwman BAM; Iftekhar A; Katainen R; Cajuso T; Crosetto N; Orozco M; Aaltonen LA; Meyer TF
Article: NATURE COMMUNICATIONS. 2019;10(1):4732

Zhang X; Garnerone S; Simonetti M; Harbers L; Nicos M; Mirzazadeh R; Venesio T; Sapino A; Hartman J; Marchio C; Bienko M; Crosetto N
Article: CELL REPORTS. 2019;29(3):560-572.e4

Hazan I; Monin J; Bouwman BAM; Crosetto N; Aqeilan RI
Article: MOLECULAR CELL. 2019;75(2):267-283.e12

Gothe HJ; Bouwman BAM; Gusmao EG; Piccinno R; Petrosino G; Sayols S; Drechsel O; Minneker V; Josipovic N; Mizi A; Nielsen CF; Wagner E-M; Takeda S; Sasanuma H; Hudson DF; Kindler T; Baranello L; Papantonis A; Crosetto N; Roukos V
Article: NATURE GENETICS. 2019;51(6):1011-1023

Dellino GI; Palluzzi F; Chiariello AM; Piccioni R; Bianco S; Furia L; De Conti G; Bouwman BAM; Melloni G; Guido D; Giaco L; Luzi L; Cittaro D; Faretta M; Nicodemi M; Crosetto N; Pelicci PG
Article: NATURE COMMUNICATIONS. 2019;10(1):1636

Gelali E; Girelli G; Matsumoto M; Wernersson E; Custodio J; Mota A; Schweitzer M; Ferenc K; Li X; Mirzazadeh R; Agostini F; Schell JP; Lanner F; Crosetto N; Bienko M
Article: GENOME BIOLOGY. 2019;20(1):28

Ballinger TJ; Bouwman BAM; Mirzazadeh R; Garnerone S; Crosetto N; Semple CA
Article: CELL. 2018;173(4):879-893.e13

Kim C; Gao R; Sei E; Brandt R; Hartman J; Hatschek T; Crosetto N; Foukakis T; Navin NE
Article: METHODS IN MOLECULAR BIOLOGY. 2018;1672:167-194

Mirzazadeh R; Kallas T; Bienko M; Crosetto N
Article: METHODS IN MOLECULAR BIOLOGY. 2018;1766:303-333

Gelali E; Custodio J; Girelli G; Wernersson E; Crosetto N; Bienko M
Article: COMMUNICATIONS BIOLOGY. 2018;1:209

Wu C; Simonetti M; Rossell C; Mignardi M; Mirzazadeh R; Annaratone L; Marchio C; Sapino A; Bienko M; Crosetto N; Nilsson M
Article: COMMUNICATIONS BIOLOGY. 2018;1:181

Biernacka A; Zhu Y; Skrzypczak M; Forey R; Pardo B; Grzelak M; Nde J; Mitra A; Kudlicki A; Crosetto N; Pasero P; Rowicka M; Ginaiski K
Article: NATURE COMMUNICATIONS. 2017;8(1):374

Asim M; Tarish F; Zecchini HI; Sanjiv K; Gelali E; Massie CE; Baridi A; Warren AY; Zhao W; Ogris C; McDuffus L-A; Mascalchi P; Shaw G; Dev H; Wadhwa K; Wijnhoven P; Forment JV; Lyons SR; Lynch AG; O'Neill C; Zecchini VR; Rennie PS; Baniahmad A; Tavare S; Mills IG; Galanty Y; Crosetto N; Schultz N; Neal D; Helleday T
Article: NATURE COMMUNICATIONS. 2017;8(1):228

Gao R; Kim C; Sei E; Foukakis T; Crosetto N; Chan L-K; Srinivasan M; Zhang H; Meric-Bernstam F; Navin N
Article: NATURE BIOTECHNOLOGY. 2017;35(8):789-792

Gao L; Cox DBT; Yan WX; Manteiga JC; Schneider MW; Yamano T; Nishimasu H; Nureki O; Crosetto N; Zhang F
Article: NATURE COMMUNICATIONS. 2017;8:15656

Iannelli F; Galbiati A; Capozzo I; Nguyen Q; Magnuson B; Michelini F; D'Alessandro G; Cabrini M; Roncador M; Francia S; Crosetto N; Ljungman M; Carninci P; di Fagagna FD
Article: NATURE COMMUNICATIONS. 2017;8:15058

Yan WX; Mirzazadeh R; Garnerone S; Scott D; Schneider MW; Kallas T; Custodio J; Wernersson E; Li Y; Gao L; Federova Y; Zetsche B; Zhang F; Bienko M; Crosetto N
Article: BLOOD. 2017;129(18):2479-2492

Shi W; Vu T; Boucher D; Biernacka A; Nde J; Pandita RK; Straube J; Boyle GM; Al-Ejeh F; Nag P; Jeffery J; Harris JL; Bain AL; Grzelak M; Skrzypczak M; Mitra A; Dojer N; Crosetto N; Cloonan N; Becherel OJ; Finnie J; Skaar JR; Walkley CR; Pandita TK; Rowicka M; Ginalski K; Lane SW; Khanna KK
Article: ONCOTARGET. 2017;8(12):18680-18698

Annaratone L; Simonetti M; Wernersson E; Marchio C; Garnerone S; Scalzo MS; Bienko M; Chiarle R; Sapino A; Crosetto N
Article: NATURE BIOTECHNOLOGY. 2016;34(8):852-856

Mooijman D; Dey SS; Boisset J-C; Crosetto N; van Oudenaarden A
Article: NATURE MEDICINE. 2016;22(3):270-277

Ambrogio C; Gomez-Lopez G; Falcone M; Vidal A; Nadal E; Crosetto N; Blasco RB; Fernandez-Marcos PJ; Sanchez-Cespedes M; Ren X; Wang Z; Ding K; Hidalgo M; Serrano M; Villanueva A; Santamaria D; Barbacid M
Article: BLOOD CANCER JOURNAL. 2014;4(10):e249

Barreca A; Martinengo C; Annaratone L; Righi L; Chiappella A; Ladetto M; Demurtas A; Chiusa L; Stacchini A; Crosetto N; van Oudenaarden A; Chiarle R
Article: JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE. 2014;8(5):396-406

Ceccarelli G; Benedetti L; Galli D; Prè D; Silvani G; Crosetto N; Magenes G; Cusella De Angelis MG
Article: CELL REPORTS. 2014;6(1):18-23

Semrau S; Crosetto N; Bienko M; Boni M; Bernasconi P; Chiarle R; van Oudenaarden A
Article: NATURE METHODS. 2013;10(4):361-365

Crosetto N; Mitra A; Silva MJ; Bienko M; Dojer N; Wang Q; Karaca E; Chiarle R; Skrzypczak M; Ginalski K; Pasero P; Rowicka M; Dikic I
Article: NATURE METHODS. 2013;10(2):122-124

Bienko M; Crosetto N; Teytelman L; Klemm S; Itzkovitz S; van Oudenaarden A
Article: ONCOGENE. 2012;31(43):4599-4608

Pareja F; Ferraro DA; Rubin C; Cohen-Dvashi H; Zhang F; Aulmann S; Ben-Chetrit N; Pines G; Navon R; Crosetto N; Köstler W; Carvalho S; Lavi S; Schmitt F; Dikic I; Yakhini Z; Sinn P; Mills GB; Yarden Y
Article: CARDIOVASCULAR TOXICOLOGY. 2012;12(2):135-142

Garrone O; Crosetto N; Lo Nigro C; Catzeddu T; Vivenza D; Monteverde M; Merlano M; Feola M
Article: JOURNAL OF PROTEOME RESEARCH. 2011;10(5):2140-2153

Pfeiffer MJ; Siatkowski M; Paudel Y; Balbach ST; Baeumer N; Crosetto N; Drexler HCA; Fuellen G; Boiani M
Article: ANNALS OF ONCOLOGY. 2011;22(3):712-717

Merlano M; Russi E; Benasso M; Corvò R; Colantonio I; Vigna-Taglianti R; Vigo V; Bacigalupo A; Numico G; Crosetto N; Gasco M; Lo Nigro C; Vitiello R; Violante S; Garrone O
Article: CELL. 2010;143(5):677-681

Ikeda F; Crosetto N; Dikic I
Article: MOLECULAR CELL. 2010;37(3):396-407

Bienko M; Green CM; Sabbioneda S; Crosetto N; Matic I; Hibbert RG; Begovic T; Niimi A; Mann M; Lehmann AR; Dikic I
Article: INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY. 2010;54(11-12):1649-1657

Pfeiffer MJ; Balbach ST; Esteves TC; Crosetto N; Boiani M
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2008;283(50):35173-35185

Crosetto N; Bienko M; Hibbert RG; Perica T; Ambrogio C; Kensche T; Hofmann K; Sixma TK; Dikic I
Article: BLOOD. 2007;110(3):1004-1012

Sargin B; Choudhary C; Crosetto N; Schmidt MHH; Grundler R; Rensinghoff M; Thiessen C; Tickenbrock L; Schwäble J; Brandts C; August B; Koschmieder S; Bandi SR; Duyster J; Berdel WE; Müller-Tidow C; Dikic I; Serve H
Article: EUROPEAN JOURNAL OF CANCER. 2006;42(18):3095-3102

Dikic I; Crosetto N; Calatroni S; Bernasconi P
Article: NATURE CELL BIOLOGY. 2006;8(2):163-169

Hoeller D; Crosetto N; Blagoev B; Raiborg C; Tikkanen R; Wagner S; Kowanetz K; Breitling R; Mann M; Stenmark H; Dikic I
Article: SCIENCE. 2005;310(5755):1821-1824

Bienko M; Green CM; Crosetto N; Rudolf F; Zapart G; Coull B; Kannouche P; Wider G; Peter M; Lehmann AR; Hofmann K; Dikic I
Article: JOURNAL OF BIOLOGICAL CHEMISTRY. 2004;279(31):32786-32795

Kowanetz K; Crosetto N; Haglund K; Schmidt M; Heldin C-H; Dikic I
Article: LEUKEMIA. 2003;17(11):2107-2112

Bernasconi P; Cavigliano PM; Boni M; Calatroni S; Klersy C; Giardini I; Rocca B; Crosetto N; Caresana M; Lazzarino M; Bernasconi C
Article: BRITISH JOURNAL OF HAEMATOLOGY. 2002;118(2):545-549

Bernasconi P; Cavigliano PM; Boni M; Astori C; Calatroni S; Giardini I; Rocca B; Caresana M; Crosetto N; Lazzarino M; Bernasconi C
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Corrigendum: NATURE COMMUNICATIONS. 2024;15(1):3390

Chen JP; Diekmann C; Wu H; Chen C; Della Chiara G; Berrino E; Georgiadis KL; Bouwman BAM; Virdi M; Harbers L; Bellomo SE; Marchio C; Bienko M; Crosetto N
Editorial comment: MOLECULAR CELL. 2024;84(1):12-13

Georgiades E; Crosetto N; Bienko M
Editorial comment: NATURE METHODS. 2023;20(5):641-642

Jimenez C; Crosetto N
Review: FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY. 2023;11:1303862

Della Chiara G; Jimenez C; Virdi M; Crosetto N; Bienko M
Review: TRENDS IN GENETICS. 2022;38(10):1062-1075

Bouwman BAM; Crosetto N; Bienko M
Editorial comment: TUMORI. 2020;106(5):427-431

Garrone O; Denaro N; Ruatta F; Vanella P; Granetto C; Vandone AM; Occelli M; Cauchi C; Ricci V; Fea E; Di Costanzo G; Colantonio I; Crosetto N; Merlano MC
Review: FRONTIERS IN ONCOLOGY. 2020;10:569202

Nicos M; Krawczyk P; Crosetto N; Milanowski J
Review: FRONTIERS IN GENETICS. 2020;11:33

Crosetto N; Bienko M
Review: GENES. 2018;9(12):E632-632

Bouwman BAM; Crosetto N
Corrigendum: METHODS IN MOLECULAR BIOLOGY. 2018;1766:E1

Gelali E; Custodio J; Girelli G; Wernersson E; Crosetto N; Bienko M
Review: NATURE REVIEWS GENETICS. 2015;16(1):57-66

Crosetto N; Bienko M; van Oudenaarden A
Editorial comment: INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY. 2008;52(7):801-809

Adjaye JA; Byskov AG; Cibelli JB; De Maria R; Minger S; Sampaolesi M; Testa G; Verfaillie C; Zernicka-Goetz M; Schöler H; Boiani M; Crosetto N; Redi CA
Other: EUROPEAN JOURNAL OF HISTOCHEMISTRY. 2008;52(1):79-81

Boiani M; Crosetto N; Redi CA
Review: MOLECULAR CANCER RESEARCH. 2006;4(12):899-904

Crosetto N; Bienko M; Dikic I
Review: FEBS LETTERS. 2005;579(15):3231-3238

Crosetto N; Tikkanen R; Dikic I

̽��ѡ

About me

Research

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̽����ѡ

Nicola Crosetto

About me

Research

Articles

All other publications

News from KI

Events from KI

̽��ѡ