Victoria Menendez Benito
Adjunkt
E-postadress: victoria.menendez.benito@ki.se
ö: ,
Postadress: H5 Laboratoriemedicin, H5 Klin Kemi, 141 52 Huddinge
Om mig
- I completed my PhD at ̽ѡ in 2006. After that, I did a
postdoc at the labs of Prof. Neefjes and Prof. van Leeuwen at The Netherlands
Cancer Institute. Since 2014, I am a PI at the department of Biosciences and
Nutrition at KI.
*Doctor of Philosophy (PhD) (2006)* “The ubiquitin-proteasome system
during proteotoxic stress”, ̽ѡ, Stockholm, Sweden
*Bachelor of Biochemistry and Molecular Biology (2000)*, University of
Oviedo, Oviedo, Spain
*Bachelor of Chemistry (1998)*, University of Oviedo, Oviedo, Spain
Forskningsbeskrivning
- I am interested in understanding the basic principles that allow cells to
partition their chromosomes, organelles, and proteins during cell division. I
use budding yeast as a model organism and combine classical genetics,
microscopy, and cell biology. My current research lines are:
* *Molecular mechanisms of chromosome congression. *During cell division,
sister chromatids align at either side of the spindle equator. This
process, known as chromosome congression, contributes to the equal
segregation of chromosomes. To achieve chromosome congression requires the
coordinated action of microtubule regulatory proteins. In my lab, we have
recently discovered that the plus-end tracking protein Bik1 (the budding
yeast homolog of CLIP-170) plays an essential role in chromosome
congression. We are investigating the molecular mechanisms of Bik1 and its
interplay with kinesins and other microtubule-binding proteins.
* *Asymmetries of the budding yeast spindle pole bodies (SPB).* The
centrosome duplicates in a semi-conservative manner, each cell division
generates two centrosomes that differ in age, composition, and function.
In budding yeast, mother and daughter SPBs (the functional equivalent of
centrosomes) have different ages. In my laboratory, we are searching for
age-dependent post-translational modifications of the SPBs and evaluating
their role in regulating cell cycle progression.
* *Protein inheritance during asymmetric cell division. *Asymmetric
dividing cells use their polarity to unequally segregate cellular
components (including organelles, proteins and RNAs) between daughter
cells. This mechanism allows cells to propagate fitness and specific
traits to individual progeny. However, a global view of the proteins with
asymmetric inheritance and their link with lifespan is still lacking,
partly because birth-dating and following proteins at cellular resolution
is technically difficult. In my lab, we address this challenge using a
genetic method named recombination induced tag exchange (RITE), which
allows visualizing protein inheritance in budding yeast by microscopy.
Undervisning
- Along with my research activities, I contribute to the education of students
at both undergraduate and graduate levels.
My teaching style is influenced by my experience as a researcher and
supervisor. I have trained one student technician; seven master's students,
and I am currently the principal supervisor of two doctoral students.
Overall, I teach diverse topics in the classroom, including cell biology
(cell cycle), the fundaments of laboratory methods (molecular biology, gene
editing, real-time PCR, high-throughput microscopy, and image analysis) and
scientific communication. I regularly teach in the courses "Applied
Communication in Biosciences" (Master's program in Biomedicine) and
"Genetics, genomics, and functional genetics" (Biomedicine Bachelor's
Program). Furthermore, I am an evaluator of the project proposal and examiner
of the oral presentations in the "Degree Project Course" (Master's program in
Biomedicine). Besides, I have directed a PhD course, The Cell Cycle", and
frequently lecture in other PhD courses at ̽ѡ.
To bring effectiveness and meaning to the teaching and learning experience, I
focus on three goals:
* Promoting active learning by designing learning activities where the
students can directly apply the concepts and theory.
* Training the students in critical thinking skills by using inquiry-based
instruction.
* Fostering learning through effective communication and collaboration by
designing learning activities based on group work.
Artiklar
- Journal article: BIOLOGY OPEN. 2022;11(11):bio059565
- Article: MOLECULAR BIOLOGY OF THE CELL. 2022;33(5):br7
- Article: JOURNAL OF CELL SCIENCE. 2017;130(19):3336-3346
- Article: GENOME RESEARCH. 2015;25(6):872-883
- Article: JOURNAL OF CELL SCIENCE. 2015;128(1):109-117
- Article: G3-GENES GENOMES GENETICS. 2013;3(8):1261-1272
- Article: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2013;110(1):175-180
- Article: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2010;107(1):64-68
- Article: MOLECULAR AND CELLULAR BIOLOGY. 2009;29(7):1774-1785
- Article: CANCER RESEARCH. 2007;67(20):10078-10086
- Article: MOLECULAR CELL. 2007;26(2):175-188
- Article: CHEMISTRY AND BIOLOGY. 2006;13(11):1217-1226
- Article: HUMAN MOLECULAR GENETICS. 2005;14(19):2787-2799
- Article: NATURE BIOTECHNOLOGY. 2003;21(8):897-902
Alla övriga publikationer
- Preprint: BIORXIV. 2021
- Editorial comment: IMMUNITY. 2007;26(1):1-3
- Letter: FASEB JOURNAL. 2006;20(7):1027-1028
- Doctoral thesis: 2006
- Meeting abstract: EUROPEAN NEUROPSYCHOPHARMACOLOGY. 2006;16:S183
- Review: RECOMBINANT PROTEIN EXPRESSION: PROKARYOTIC HOSTS AND CELL-FREE SYSTEMS. 2005;399:490-511
Forskningsbidrag
- Kartläggning av jästproteomets nedärvning i syfte att utforska mekanismerna bakom åldrande och föryngring̽ѡ - KID funding1 January 2024
- Primär cilie-centrosom cyckeln i njursjukdomStiftelsen för Strategisk Forskning1 January 2021 - 1 January 2022
- Mapping the inheritance of the yeast proteome to discover mechanisms of ageing and rejuvenationStiltefsen för Internationalisering an högre utbildning och forskning1 January 2019 - 1 January 2026
- Kartläggning av jästproteomets nedärvning isyfte att utforska mekanismerna bakom åldrande och föryngringձٱԲ첹å1 January 2018 - 1 January 2021
- Att utveckla ett jästbiblitotek för systematisk analysis av in vivo protein dynamikCarl Tryggers Stiftelse för Vetenskaplig Forskning1 January 2017 - 1 January 2018
- Rollen av centrosomer i asymmetrisk celldelningÅke Wibergs Stiftelse1 January 2016 - 1 January 2017
- Rollen av centrosomer i asymmetrisk celldelningÅke Wibergs Stiftelse1 January 2015 - 1 January 2016
- Att avkoda rollen för centrosomer i asymmetrisk celldelning̽ѡ _ KID funding
- Proteomik och storskalig screening av jäst mutanter för att förstå centrosom polarisation; en nyckelprocess för asymmetrisk celldelningձٱԲ첹å
ԲäԾԲ
- Adjunkt, Laboratoriemedicin, ̽ѡ, 2022-
Examina och utbildning
- MEDICINE DOKTORSEXAMEN, Institutionen för cell- och molekylärbiologi, ̽ѡ, 2006
- Magisterexamen i Biokemi och Molekylärbiologi, University of Oviedo, 2000
- Magister i Kemi, University of Oviedo, 1998
Handledning
- Jana Lalakova, 2015
- Alexander Julner, Microtubule and chromosome dynamics during mitosis in budding yeast
- Marjan Abbasi, Microtubule and Spindle Pole Body regulation in the budding yeast mitotic spindle
Gästforskning och resestipendier
- Postdoktor Forskningsstipendiat, Antoni van Leeuwenhoek Hospital, 2006-2012
Utvärdering av avhandlingsarbete
- Laura Matellán Fernández, Examination board member, Andalusian Molecular Biology and Regenerative Medicine Centre, Study of asymmetries associated with the mitotic spindle of Saccharomyces cerevisiae, 2020
- Anna Bosch Commas, Examination board member, Genetics, University of Barcelona, USP25 deubiquitylating enzyme: substrate search and structure-function relationship, 2017
- Helena Silva Cascales, Examination board member, CMB, ̽ѡ, Cyclins on the move: a time and a place for Cyclin A2 and cycling B1 in the human cell cycle, %20Silva_Cascales.pdf?sequence=1&isAllowed=y, 2016
- Sjoerd van Devender, Examination board member, Leiden University, Tracking the big ones: Novel dynamics of organelles and micromolecular complexes during cell division and aging, , 2015